whatman schleicher & schuell公司杂交膜选择指南-凯发官方首页
whatman schleicher & schuell公司杂交膜选择指南
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protran
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optitran
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nytran
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nnytran
spc
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westran s
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westran
cs
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膜的种类
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100%纯硝酸纤维素
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加强型硝酸纤维素
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带中等量正电荷的尼龙
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带较高量正电荷的尼龙
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pvdf
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pvdf
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结合能力μg/cm2
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75-110
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75-90
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>400
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>400
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50-100
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50-100
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孔径大小μm
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0.45（ba85）
0.20（ba83）
0.10（ba79）
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0.45（ba-s85）
0.20（ba-s83）
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0.45
0.20
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0.45
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0.20
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0.45
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可使用的固定方法
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baking(vacuum oven, 80℃)
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n/a
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n/a
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uv cross-linking
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n/a
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n/a
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drying
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(蛋白)
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(蛋白)
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reprobing
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有限
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yes
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yes
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yes
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yes
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yes
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应用
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southern blotting
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n/a
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n/a
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northern blotting
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n/a
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n/a
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western blotting
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n/a
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protein sequncing
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-
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-
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-
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-
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macroarraying
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(protein)
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(protein)
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(dna)
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(dna)
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(protein)
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(protein)
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转移方法
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semidry electroblotting
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tank electroblotting
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vacuum blotting
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capillary blotting
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alkaline method
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-
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-
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-
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-
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检测方法
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colorimetric
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clemiluminescent
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isotopic
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staining
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(ba85,ba83)
(ba79)
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n/a
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n/a
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fluorescent
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-
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-
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-
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-
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-
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：推荐 ：满足 -；不推荐 n/a：不适合
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